Ribozyme-based insulator parts buffer synthetic circuits
In particular, pTAC had a 27-nt leader sequence, whereas pBAD produced a 5-nt leader (Supplementary Fig. 1). To test the importance of these leader sequences, we used the pLlacO-1 promoter 25, which is induced by IPTG, but in which the operator has been moved upstream of the transcription site (Supplementary Fig. 1). ...
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