Team:British Columbia/pBAD - 2009.igem.org

Link: http://2009.igem.org/Team:British_Columbia/pBAD

FREEMutagenesis of pBAD promoter sequence to create a stronger promoter (Strong pBAD) and a weaker promoter (Weak pBAD). Quantification of mutant promoter-driven RFP fluorescence. BioBrick submission. pBAD Mutagenesis. A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated from the wild type to form ...

Actived: Thursday Oct 1, 2020

Upstream repression and CRP stimulation of the Escherichia

Link: https://www.sciencedirect.com/science/article/pii/0022283684904303

FREEThis mutation increases the homology of PBAD to the 5 promoter consensus sequence (Hawley & McClure. 1983). From the sizing of 40-nucleotide run-off transcripts made in vitro from the wild type and mutant promoters, transcription of both promoters was found to initiate from the same position 1 nucleotide (not shown). In the absence of AraC protein in vivo, the AraC-independent promoter (cip-5 ...

Actived: Monday Sep 28, 2020

Ribozyme-based insulator parts buffer synthetic circuits

Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3914141/

FREEIn particular, pTAC had a 27-nt leader sequence, whereas pBAD produced a 5-nt leader (Supplementary Fig. 1). To test the importance of these leader sequences, we used the pLlacO-1 promoter 25, which is induced by IPTG, but in which the operator has been moved upstream of the transcription site (Supplementary Fig. 1). ...

Actived: Sunday Jan 22, 2017

How To Predict Football Results: An Ultimate Tool For Any

Link: https://alphasportsbetting.com/sports-betting-tactics/how-to-predict-football-results

FREEThe football prediction site is proven to predict 76% of the accurate bet based on a wide variety of leagues like the Bundesliga and the English Premier League. This is a great tool that will aid both the quality and value of your bets by sifting through the current football trends and different team dynamics for every single match. Betegy has worked on improving its smart technology for ...

Actived: Monday Oct 19, 2020

Football Predictions | FootballPredictions.com

Link: https://footballpredictions.com/footballpredictions/

FREEA lot of viewers also like to register their own predictions and football tips on games, and as such, this page is going to be of good use to anyone who fits into that category. This site, and page specifically, allows you to read through all of the predictions for every single major game on a day to day basis. If there are no primary games taking place on the day that you happen to stumble ...

Actived: Monday Oct 19, 2020

Cell-free prediction of protein expression costs for

Link: https://www.nature.com/articles/s41467-018-03970-x

FREETwo constructs (B3-1 str and B10-2 str, both containing promoter BBa_J23100) were obtained by PCR amplification designed to introduce mutations in the weak promoter sequence BBa_J23113. ...

Actived: Sunday Aug 23, 2020

Plasmids 101: Inducible Promoters - Addgene

Link: https://blog.addgene.org/plasmids-101-inducible-promoters

FREEIn the OFF state, the promoter is inactive because a bound repressor protein actively prevents transcription. Once an inducer binds the repressor protein, the repressor protein is removed from the DNA. With the repressor protein absent, transcription is turned ON. Types of inducible promoters. Chemical agents, temperature, and light are all examples of factors that can lead to the induction of ...

Actived: Monday Oct 19, 2020

Colicin Z, a structurally and functionally novel colicin

Link: https://www.nature.com/articles/s41598-019-47488-8

FREEA primosome assembly site for pColZ was identified between positions 82 and 147, and this 66 bp sequence was 98.5% identical to the phiX174 primosome assembly site identified in plasmid pHUSEC ...

Actived: Saturday May 23, 2020

Addgene: Cre-lox system

Link: https://www.addgene.org/collections/cre-lox/

FREEPromoter-regulated Cre: The promoter region defines the areas in which Cre will be expressed. Cre may be expressed globally under a broadly active promoter like CAG, or expressed only in a subset of cells under a more specific promoter (e.g. Rho-Cre is expressed in the retina). ...

Actived: Monday Oct 19, 2020

A bottom-up approach to gene regulation | Nature

Link: http://www.nature.com/nature/journal/v439/n7078/fig_tab/nature04473_F3.html

FREEThe ability to construct synthetic gene networks enables experimental investigations of deliberately simplified systems that can be compared to qualitative and ...

Actived: Thursday Jul 16, 2020